Give diagrammatic explanation of biotechnology process for the preparation of insulin?
Answers
Answer:
insulin is a protein composed of two chains, an A chain (with 21 amino acids) and a B chain (with 30 amino acids), which are linked together by sulfur atoms. Insulin is derived from a 74-amino-acid prohormone molecule called proinsulin
Explanation:
Human insulin is extracted from pancreas cells and an insulin-producing gene is isolated. ... Insert human insulin-producing gene into the bacterial plasmid vector to form the recombinant DNA of human insulin-producing gene. 4. Introduce this recombinant DNA into a bacterial cell to form the recombinant bacterium.
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Answer:
Explanation:
Biotechnology definition:
Biotechnology is science-based technology that uses cellular and biomolecular processes to create goods and services that enhance both the quality of our lives and the health of our world.
Insulin definition:
A hormone produced by the pancreatic islet cells. By delivering sugar to the cells, where it may be utilized by the body for energy, insulin regulates the amount of sugar in the blood.
Process for preparation of human insulin:
1. Pancreatic cells are used to extract human insulin, and an insulin-producing gene is discovered.
2. To create a plasmid vector, plasmid DNA is taken from a bacterium and cut with a restriction enzyme.
3. To create the recombinant DNA of the human insulin-producing gene, insert the human insulin-producing gene into the bacterial plasmid vector.
4. To create the recombinant bacterium, insert this recombinant DNA into a bacterial cell.
5. In a fermentation tank, the recombinant bacteria grow and create human insulin.
6. Insulin is refined, extracted, and packaged. After that, diabetic patients can receive an injection of it.
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