Question

give short note on denaturation and renaturation of proteins (for 6marks)

Answer 1

Denaturation is when proteins fold upon themselves because the bonds are disrupted.

causes of denaturing are :-
1. heat
2. pH change.

Heat causes the bonds in the proteins to break and destroy their structure

High pH also causes the same effect. The OH ions pull at the hydrogen bonds, and disrupt the structure...

peptide bonds of secondary and tertiary structure are destroyed

once the protein becomes denatured it can't be renatured even if peptide bonds of primary structure are present

Answer 2

Denaturation is a process in which proteins or nucleic acids lose the quaternary structure, tertiary structure and secondary structure which is present in their native state, by application of some external stress or compound such as a strong acid or base, a concentrated inorganic salt, an organic solvent

renaturation does not occur