Give some information about
1) restriction enzymes
2) cloning vectors
3) ori
Answers
Answer:
Restriction Enzymes :
The restriction Enzymes and it's corresponding methylase constitute the restriction modification system of a bacterial species . Traditionally four types of restriction Enzymes are recognized , designed I , II ,III,IV which differ primarily in structure , cleavage site , specificity , and cofactors .
Cloning Vectors :
A cloning vector is a small piece of DNA that can be stably maintained in a organism , and into which a foreign DNA fragment can be inserted for cloning purposes . The cloning vector may be DNA taken from a virus , or the cell of s higher organism , or it may be the plasmid of a bacterium .
Ori :
Section of DNA sequence which is recognised by a cell 's DNA replication protiens , allowing initiation of new DNA synthesis .
Answer:
Ori - It is a genetic sequence that acts as the initiation site or the origin site for the replication of DNA. Any fragment of DNA, when linked to the ori region, can be initiated to replicate.
Restriction site - It is the recognition site for restriction enzymes (such as EcoRI, Hind III, PvuI, BamHI, etc.). Recognition sites are the genetic sequences where the restriction enzymes cut the DNA segments.