Biology, asked by survirawat350, 10 months ago

Give the operating steps of autoclave

Answers

Answered by chrislermessiah24
0

Answer:

Operating Autoclave  

• Check to be sure that the water reservoir is filled (approximately 1” from top), and if not, fill with distilled water.

• Choose appropriate cycle ( e.g. fluid, dry etc) for the material.

• Set appropriate temperature for the cycle.  

• Turn the autoclave to FILL and wait until the water in the bottom of the autoclave covers the fill plate.

• Turn the autoclave to STERILIZE and load autoclave

• Close and lock door

• A steam cycle is approximately 40 minutes if the autoclave is cold and approximately 20 minutes if it is already warmed from a previous steam cycle.

• When cycle is complete, turn autoclave to VENT and remain there until pressure drops. The heat stays on during the venting and without the steam plastics will melt.

• Turn the autoclave to POWER OFF.

• Do not attempt to open the door while autoclave is operating.  

• The manuals for operation of the autoclave are located on the wall behind the autoclave.

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