Question

Glycerine is added on the stained specimen to be observed. What is the reason behind this. a) to observe the specimen clearly b) to prevent drying up of the specimen c) to fix the observation material on the stage​

Answer 1

Answer:

option B is correct ans for this

Answer 2

Both the option a and b are the correct solutions.

• Glycerine is widely used to prepare a temporary mount of the specimens in order to prevent drying up and also to obtain a clear vision under the microscope.
• Glycerine is isotonic compared with cell sap, therefore cells will not shrink or swell. The cell will remain in its natural state.
• Glycerine always makes mounted specimen moist and thus prevents it's from drying out
• The biological cells require mounting media when placed in a glass slide in order to fix the glass slide. Since Glycerine has a high refractive index and high density, it will make the specimen more clear and visible by refracting the light source more to the specimen.