Question

Ground sub of mitochondria

Answer 1

HEY MATE ---

$\huge\underline\mathfrak\red{AnSweR}$

✒️ INTRODUCTION ✒️

✍️Photoreceptors are high energy-consuming cells. Their functionality therefore relies heavily on mitochondrial activities. Failure of mitochondria to adapt to metabolically challenging conditions has been implicated in many photoreceptor diseases. We are interested in identifying strategies adopted by photoreceptor mitochondria in the hibernating ground squirrel retina which enable it to cope with drastic changes in metabolic conditions and yet emerge without any evident damage to their tissues or organ systems. Thus, we set out to investigate changes in mitochondrial structure, enzyme activity and fusion/fission dynamics in these animals.

✒️ METHODS ✒️

✍️Retinal tissues from both hibernating and awake animals were imaged using serial blockface scanning electron microscopy with an x-y resolution of 7nm and z resolution of 30nm. Individual mitochondria were traced manually and analyzed using Reconstruct software. BN-PAGE was used to measure Complex V activity in the retinal mitochondria, while spectrophotometric cytochrome quantitation was used to quantify Cytochrome...

Answer 2

Photoreceptors are high energy-consuming cells. Their functionality therefore relies heavily on mitochondrial activities. Failure of mitochondria to adapt to metabolically challenging conditions has been implicated in many photoreceptor diseases. We are interested in identifying strategies adopted by photoreceptor mitochondria in the hibernating ground squirrel retina which enable it to cope with drastic changes in metabolic conditions and yet emerge without any evident damage to their tissues or organ systems. Thus, we set out to investigate changes in mitochondrial structure, enzyme activity and fusion/fission dynamics in these animals.