Question

Heat and chemical treatments in adult cyprinus carpio (pisces cypriniformes) rapidly produce sterile gonads.

Answer 1

Adult common carp Cyprinus carpio reared at 38 °C water temperature for 10 weeks with 2-week interval injection of cytotoxic drug busulfan(40 mg/kg; 5 doses in total) produce sterile gonads.

•

Adverse effects of drug on the animal health was not observed during the study, even after injecting five doses of 40 mg/kg busulfan.

•

The change of gonads colouration into black appears to be an additional useful index to measure the sterility in teleost fishes.

•

The treatment protocol for induction of sterility in male and female adult common carp described here will have profound implication for germ cell transplantation in adult fishes.