Biology, asked by kaish282, 1 year ago

Heat inactivation of restriction enzymes before ligation

Answers

Answered by dalvividya

Answer:

Heat inactivation is a convenient method for stopping a restriction endonuclease reaction. Incubation at 65°C for 20 minutes inactivates the majority of restriction endonucleases that have an optimal incubation temperature of 37°C.

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