Hello guys..
How PCR (Polymerase chain reaction) can be used to detect mutations?
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Answers
In my experience, we can develop a PCR primer set or more to amplify mutation genes.
1- we collect the nucleotide sequence of both original gene and mutation genes.
2- decide the primer at that point of mutation. So that designed primer would be able to amplify only the gene that get mutated. We also decided a primer set for amplify the same position to the original gene.
3- There should be 1 positive control, for 1 positive original gene and mutation genes
4- i was success to use in my study, but i recommend you to consult with other molecular biologist
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Answer:
PCR helps focus on the actual segment of DNA that is of interest, rather than the whole genome. From a small genetic sample, the genotypes can now be determined, and as a result, many genetic disorders can be detected, diagnosed and monitored.