Biology, asked by hardikrakholiya21, 10 months ago

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◆While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

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Answered by shnehashneha5962

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Answered by Avni2348

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Denaturation is process in which double helical DNA unwinds at high temperature to produce single stranded DNA templates. If the denaturation step is missed, the single stranded template needed for annealing of primars will not be produced. Hence, PCR reaction will not proceed further.

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