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Answers
Tests to conform to the presence of Protein
Aim
To perform a simple test to identify the presence of proteins in the given plant and animals food sample.
Materials Required
StirrerDropperWater bathDistilled waterBunsen burnerTest tube holderClean and dried test tubesFood sample – Milk, Apple, Paneer, Butter, Cheese, etc.
If you are using a solid sample like paneer, or apple, first crush or grind into a smooth paste using mortar and pestle.
Chemical required
Nitric acidSulfuric acidSodium nitriteMercuric sulfatePyridine solutionNinhydrin reagentSodium hydroxideCopper sulfate solutionBiuret reagent solution or solution of copper sulfate and caustic soda.
Procedure
(a) Biuret Test:
Take a cleaned and dried test tube.Add the food samples of your choice into the test tubes.Add 2ml of sodium hydroxide and 5 to 6 drops of copper sulfate solution to it.Shake the test tube gently to mix the ingredients thoroughly and allow the mixture to stand for 4 – 5 minutes.If there is the appearance of bluish- violet color, it indicates the presence of protein.
(b) Xanthoproteic Test:
Take a cleaned and dried test tube.Add the food samples of your choice into the test tubes.Add a few drops of concentrated sulfuric acid and shake the test tube.Heat the test tube gently on a Bunsen burner.If there is a formation of yellow precipitate, then the presence of protein is confirmed.