Hepatoprotective activity of medicinal plants at molecular level
Answers
Answered by
0
Abstract
Objective:
To evaluate the hepatoprotective properties of Otostegia persica (O. persica) ethanol extract on carbon tetrachloride-induced liver damage in rats.
Materials and Methods:
Fifty adult male Wistar rats were randomly divided into five groups. Group I served as normal control and was given only olive oil intraperitoneally (i.p.). Group II, III, IV, and V were administered CCl4mixed with olive oil 1:1 (1 ml/kg) i.p., twice a week for 8 weeks. Group II was maintained as CCl4-intoxicated control (hepatotoxic group). Group III, IV, and V received O. persicaextract at a dose of 40, 80, and 120 mg/kg for 8 weeks every 48 h orally, respectively. Biochemical parameters including aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), albumin (ALB), total protein (TP), and lipid peroxidation marker (Malonaldialdehyde, (MDA) were determined in serum. After 8 weeks, animals were sacrificed, livers dissected out, and evaluated for histomorphological changes.
Results:
The administration of CCl4 increased AST, ALT, ALP, TB, and MDA in serum but it decreased TP , and ALB compared with normal control. Treatment with O. persicaextract at three doses resulted in decreased enzyme markers, bilirubin levels, and lipid peroxidation marker (MDA) and increased TP and ALB compared with CCl4 group. The results of pathological study also support the hepatoprotective effects which were observed at doses of 80 and 120 mg/kg.
Conclusion:
The results of the present study indicate that ethanol extract of O. persica may have hepatoprotective effect which is probably due to its antioxidant property.
Key Words: Hepatoprotective effect, Otostegia persica, Liver injury, Carbon Tetrachloride, Antioxidant property
Introduction
Liver diseases are serious health problems. The effectiveness of treatments such as interferon, colchicine, penicillamine, and corticosteroids are inconsistent at best and the incidence of side-effects profound. In the absence of reliable liver protective drugs in allopathic medical practices, herbs play an important role in the management of various liver disorders. A number of plants have shown hepatoprotective property (Luper, 1998 ▶).
Preparation of extracts
The aerial parts of the O. persica were collected from Firozabad, Fars, Iran. A voucher specimen was deposited in the herbarium of Kohkyloyeh and Boyerahmad Agricultural Research Center. The O. persica was powdered in an electrical grinder. The extraction was carried out through the maceration of dry plant powder in ethanol 70% for 48 h at incubation temperature. Then, it was extracted and ethanol was evaporated by rotary evaporator at 40-50 ºC.
Objective:
To evaluate the hepatoprotective properties of Otostegia persica (O. persica) ethanol extract on carbon tetrachloride-induced liver damage in rats.
Materials and Methods:
Fifty adult male Wistar rats were randomly divided into five groups. Group I served as normal control and was given only olive oil intraperitoneally (i.p.). Group II, III, IV, and V were administered CCl4mixed with olive oil 1:1 (1 ml/kg) i.p., twice a week for 8 weeks. Group II was maintained as CCl4-intoxicated control (hepatotoxic group). Group III, IV, and V received O. persicaextract at a dose of 40, 80, and 120 mg/kg for 8 weeks every 48 h orally, respectively. Biochemical parameters including aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), albumin (ALB), total protein (TP), and lipid peroxidation marker (Malonaldialdehyde, (MDA) were determined in serum. After 8 weeks, animals were sacrificed, livers dissected out, and evaluated for histomorphological changes.
Results:
The administration of CCl4 increased AST, ALT, ALP, TB, and MDA in serum but it decreased TP , and ALB compared with normal control. Treatment with O. persicaextract at three doses resulted in decreased enzyme markers, bilirubin levels, and lipid peroxidation marker (MDA) and increased TP and ALB compared with CCl4 group. The results of pathological study also support the hepatoprotective effects which were observed at doses of 80 and 120 mg/kg.
Conclusion:
The results of the present study indicate that ethanol extract of O. persica may have hepatoprotective effect which is probably due to its antioxidant property.
Key Words: Hepatoprotective effect, Otostegia persica, Liver injury, Carbon Tetrachloride, Antioxidant property
Introduction
Liver diseases are serious health problems. The effectiveness of treatments such as interferon, colchicine, penicillamine, and corticosteroids are inconsistent at best and the incidence of side-effects profound. In the absence of reliable liver protective drugs in allopathic medical practices, herbs play an important role in the management of various liver disorders. A number of plants have shown hepatoprotective property (Luper, 1998 ▶).
Preparation of extracts
The aerial parts of the O. persica were collected from Firozabad, Fars, Iran. A voucher specimen was deposited in the herbarium of Kohkyloyeh and Boyerahmad Agricultural Research Center. The O. persica was powdered in an electrical grinder. The extraction was carried out through the maceration of dry plant powder in ethanol 70% for 48 h at incubation temperature. Then, it was extracted and ethanol was evaporated by rotary evaporator at 40-50 ºC.
Similar questions