Question

Hershey and chase used the 35S isotope to label proteins. How does this isotope differ from the 32S isotope found in the greatest natural abundance, and why did they choose 35S instead of 32S

Answer 1

They used 35S because it is radioactive. So it was easily visible after centrifugation

Answer 2

The number of neutron in 35S is greater than 32S

Explanation:

• As sulfur is present in protein not DNA Hershey and chase used sulfur to label protein. Harshey and chase used 35S which is one of the isotopes of sulfur.  
• The 35S is different from 32S because 35S has a higher number of neutrons than 32S. They used 35S because this isotope is radioactive.  
• So as the 35S is radioactive it became easy for Hershey and chase to trace the radioactive sulfur. They found that the radioactive sulfur is present in the supernatant and not inside the cell which shows that protein is not genetic material.

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