Question

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what is gram staining?..


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Answer 1

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Gram Staining :-

A process to found the nature of gram weather it is positive or negative by changing colors red & voilet.

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Answer 2

what is gram staining?..

it was done by Christian Gram

he classified bacteria on the basis of cell wall thickness

there are two types of bacteria

1 Gram positive

it take crystal violet stain and iodine

amount of peptidoglycan ( constituent of cell wall) is more

thickness = 20 - 80 um

lipids are absent

mesosoms are present and prominent

rarely cause disease

ex = lactobacillus

2 gram negative bacteria

don't take stain from Crystal violet and it takes stain from safranin solution

amount of prptidoglycan is less

thickness = 10 - 20 um

lipids are present

mesosomes are absent or less prominent

generally cause disease

because = they have high resistances against antibiotics and have more power

ex = e. coli

it is enough from the neet and AIIMS point of view