High doses of caffeine interferers with the DNA replication check point mechanism in mammalian cells. Why then do you suppose the Surgeon General has not yet issued an appropriate warning to heavy coffee and cola drinkers? A typical cup of coffee (150 ml) contains 100 mg of caffeine (196 g/mole). How many cups of coffee would have to drink to reach the dose (10 mM) required to interfere with the DNA replication checkpoint mechanism?
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High doses of caffeine interfere with the DNA replication checkpoint mechanism in mammalian cells. Why do you suppose the surgeon general has not issued a warning to heavy coffee and cola drinkers?
[To answer this question, lets assume that a 150ml cup of coffee contains 100 mg of caffeine (196g/mol), and for the sake of the calculation, that you cannot measurably metabolize or excrete caffeine (which is not true) in the pweiod of one day once you ingest it. Let's also assume that the caffeine distributess itself evenly throughout the total body fluid. How many cups of coff would you have to drink in one day to reach the dose (10mM) required to interfere with DNA replication checkpoint mechanism? (A typical adult contains about 40L of water in total body fluid -- we will assume that you can excrete the water that you ingest, thus the total body fluid columer will remain constant at 40 liters.]
Since the inhibitory constructions of coffee 10mM is way higher rhat what we consume in a cup of coffee.
This 150 ml of coffee gets diluted into 40 L of body fluid and also it catabolism leads to a substantial decrease in the effective concentration of coffee.
Assuming no catabolism for 40 L of body fluids on would have to drink 784 cup of coffee to reach the inhibitory concentration
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