How a microarray is used to evaluate gene expression levels?
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The use of microarray technology to assess gene expression levels is now widespread in biology. The validation of microarray results using independent mRNA quantitation techniques remains a desirable element of any microarray experiment. To facilitate the comparison of microarray expression data between laboratories it is essential that validation methodologies be critically examined. We have assessed the correlation between expression scores obtained for 48 human genes using oligonucleotide microarrays and the expression levels for the same genes measured by quantitative real-time
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Explanation:DNA microarrays can simultaneously measure the expression level of thousands of genes within a particular mRNA sample. ... In Northern blot analysis, the amount of radioactivity is a function of the amount of probe hybridized, which reflects the amount of mRNA in the sample.
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