Question

how amplification of DNA is done by using PCR? write in brief

Answer 1

Hi Mate,

to amplify a segment of DNA using PCR, the sample is first heated so the DNA denatures, or seperates into two pieces of single stranded DNA point next, an enzyme called "taq polymerase" synthesizes, builds, two new strands of DNA, using the original strands as templates.

hope this helps you.......

Answer 2

Using PCR, a single copy (or more) of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment. ... In the first step of PCR, the two strands of the DNA double helix are physically separated at a high temperature in a process called DNA melting.