Question

How are DNA fragments separated and isolated for DNA finger printing? Explain

Answer 1

a. Isolation of DNA

b. Digestion of DNA y restriction endonucleases

c.Separation of DNA fragments through electrophoresis.

d.Transferring of separated DNA fragments to synthetic membranes such as nitrocellulose or nylon

e.Hybridization using labeled VNTR probe

f.Detection of hybridized DNA fragments by autoradiography.

Answer 2

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DNA fragments formed by the use of restriction endonucleases are separated by gel electrophoresis. (i) DNA fragments are negatively charged molecules. Thus, they move towards the anode under electric field through the medium. ... (iv) The separated bands of DNA are cut and extracted from gel piece.