How are restriction enzymes used in recombinant dna technology?
Answers
Answer:
Type II restriction enzymes have two properties useful in recombinant DNA technology. First, they cut DNA into fragments of a size suitable for cloning. Second, many restriction enzymes make staggered cuts generating single-stranded ends conducive to the formation of recombinant DNA.
Answer:
The restriction enzyme used mostly in recombinant DNA technology is the Type II Restriction enzymes. They are generally used in techniques involving DNA fragmentation like gene cloning. Eg : HindIII is commonly used restriction enzyme in RDT.
Explanation:
Restriction enzymes have the special charecteristics of recognizing and cleaving at specific DNA sequences.
These sequences are palindromes consisting of 6-8 bp in length. These enzymes are used to cut a particular target DNA fragment to generate fragments with blunt or sticky ends.
When the same enzyme is used to cut another DNA fragment(like a plasmid), it will generate complementary fragments. So our targent fragment can be easily joined with this DNA fragment(or plasmid) using DNA ligase enzyme.
This is how restriction enzymes are used in RDT.
