Question

How are some candidates of puc series used in directional cloning?

Answer 1

Explanation:

Cloning vector. Schematic representation of the pBR322 plasmid, one of the first plasmids widely used as a cloning vector. The pUC plasmid has a high copy number, contains a multiple cloning site (polylinker), a gene for ampicillin antibiotic selection, and can be used for blue-white screen.

Answer 2

Answer:

In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into host organism, (6)

Explanation:

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