how can a cell line beobtained from a cultured cell?
Answers
Answer:
Cell lines are something and primary cells are something else. you can not establish a cell line from primary cells as far as it would be a healthy cell, not require some kind of activator to continuously grow and divide. the cell lines are mainly from cancer, or transformed with some sort of genes which enables the cell behave like cancer cell, to grow without stop as fare as there is food and service. so if you isolate fibroblast from mouse they will grow nicely for few rounds.. after 5 or 6 splits the cell start to slow down and eventfully die, you have to carefully spit the cells to not let them become dense and out growth.
A cell strain is derived either from a primary culture or a cell line by the selection or cloning of cells having specific properties or characteristics which must be defined. Cell strains are cells that have been adapted to culture but, unlike cell lines, have a finite division potential.
hope it helps you