Question

how can a mixture of arginine and aspartic acid be separated by electrophoresis using a buffer solution having a pH=6•0?
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Answer 1

amino acids, as well as peptides and proteins, incorporate both acidic and basic functional groups, the predominant molecular species present in an aqueous solution will depend on the pH of the solution. In order to determine the nature of the molecular and ionic species that are present in aqueous solutions at different pH's, we make use of the Henderson-Hasselbalch Equation, written below. Here, the pKa represents the acidity of a specific conjugate acid function (HA). When the pH of the solution equals pKa, the concentrations of HA and A(-) must be equal (log 1 = 0).

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