How can one use the method of aqueous-two phase partitioning for the separation of proteins? Also suggest various efforts which may be taken to maximize protein stability during such separation.
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Aqueous two-phase partition was developed in Sweden in the late 1950s by Per Å ke Albertsson for the separation of proteins and other soluble material, and was adapted for the separation of cells by Harry Walter. The two polymers that have been almost exclusively used for cell separation are poly- (ethyleneglycol) (PEG) and dextran. When aqueous solutions of these are mixed together above certain critical concentrations, two phases are formed; the upper phase is PEG-rich and the lower phase is dextran-rich. When cells are added to the phase system, they distribute themselves (partition) between the interface and one of the bulk phases,
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