Question

how can retrovirus be used efficiently in biotechnology experiments inspite of them being pathogenic?

Answer 1

A retrovirus enters the host cell, then it uses an enzyme called reverse transcriptase to produce DNA from its RNA sequence
-the DNA from the retrovirus is then added to the host cell genome, by an enzyme called integrase
-the retrovirus then continues to infect host cells and add genetic material to them; this is why the retroviruses are useful in biotechnology to deliver genes
-also, the retrovirus is created in such a way that it does not cause disease or destroy host cells
-the scientists using the retroviruses also most likely ensure that the retrovirus cannot spread through air, to make it less contagious
-however, transferring infected host cells to an organism not infected by the retrovirus, for example, by contact with bodily fluids, most likely will spread the retrovirus
-once scientists used a retrovirus to transfer genes for resistance to HIV from rhesus monkeys to cats, and also added genes for florescent proteins to the retrovirus, to show that the retrovirus had inserted the genes into the host cells

Answer 2

The retrovirus is used in experiment due to following reason:

Explanation:

• Retrovirus has a single stranded RNA that replicates inside the cell of host by the mechanism of reverse transcription.
• The retrovirus has the ability to cause infection to the animal and human cells.
• The retrovirus is a HIV virus.
• In biotechnology, the retrovirus is used as a vector to transfer the gene of interest into the host. The retrovirus is modified into cloning vector this is done by deleting the genes that are responsible for pathogenicity.
• This play an important role in gene therapy to correct the problem of abnormal gene in the desired cell.

Learn more retrovirus:

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