Question

how can we separate the biomolecules using column chromatography​

Answer 1

Answer:

Column chromatography

This technique is used for the purification of biomolecules. On a column (stationary phase) firstly the sample to be separated, then wash buffer (mobile phase) are applied. Their flow through inside column material placed on a fiberglass support is ensured.

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Answer 2

Column chromatography

This technique is used for the purification of bio-molecules. On a column (stationary phase) firstly the sample to be separated, then wash buffer (mobile phase) are applied (Figure 1). Their flow through inside column material placed on a fiberglass support is ensured.

Ultracentrifugation. Cells, organelles , or macro-molecules in solution exposed to a centrifugal force will separate because they differ in mass, shape, or a combination of those factors. The instrument used for this process is a centrifuge. An ultra-centrifuge generates centrifugal forces of 600,000 g and more.