How can you identify a specific gene sequence from genomic DNA sequence of an organism?
Answers
Answer:
Annotation involves marking where the genes start and stop in the DNA sequence and also where other relevant and interesting regions are in the sequence
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Answer:
✳There are two types of gene prediction:
✳There are two types of gene prediction: 1. Ab initio – this technique relies on signals within the DNA sequence. It is an automated process whereby a computer is given instructions for finding genes in the sequence and is then left to find them. The computer looks for common sequences known to be found at the start and end of genes such as promoter sequences (where proteins ?bind that switch on genes), start codons ?(where the code for the gene product, RNA ?or protein, starts) and stop codons (where the code for the gene product ends).
2. Evidence-based – this technique relies on evidence beyond the DNA sequence. It involves gathering various pieces of genetic information from the transcript sequence (mRNA?), and known protein sequences of the genome. With these pieces of evidence it is then possible to get an idea of the original DNA sequence by working backwards through transcription? and translation? (reverse transcription/translation). For example, if you have the protein sequence it is possible to work out the family of possible DNA sequences it could be derived from by working out which amino acids? make up the protein and then which combination of codons could code for those amino acids and so on, until you get to the DNA sequence.
The information taken from these two prediction methods is then combined and lined up with the sequenced genome.
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