How can you microscopically distinguish bacteria from yeast?
Answers
well there are different forms of bacteria like spiral etc but yeast has a definite shape .... so it shape of an individual or the form of budding can distinguish between a bacteria and a yeast moreover bacterias are smaller in size ..... hope u found my answer helpful
Yeast cells are members of the Fungus Kingdom. They are single celled microorganisms (eukaryotic) classified under phyla Ascomycota (sac fungi) and Basidiomyota (higher fungi) both of which fall under the subkingdom Dikarya.
YEAST UNDER THE MICROSCOPE
Requirements:
Water
Yeast cake
Dropper
Glass slides and cover slips
Stains (stated below)
Yeast cake contains Saccharomyces Cerevisiae (sugar-eating fungus) and can therefore be used to obtain the yeast to observe under the microscope. The following is a procedure that can be used to prepare the specimen for observation.
Obtain yeast cake (this can be bought from bakery specialty stores or a supermarket)
Cut a small piece of yeast cake and mix with water to form a pasty texture
Add a little more water to form a solution
Using a dropper, collect and place a drop of the solution on a microscope slide
Place a microscope cover slip and observe under high power objective
Observation
WITH BRIGHTFIELD MICROSCOPY
When viewing the specimen under high magnification (1000x and above) one will see oval (egg shaped) organism, which are the yeast. It is also possible to observe the buds, which can be seen on some of the cells.
If the solution had some sugar, one will also notice some bubbles in the specimen, which are as a result of the fermentation process by the microorganisms.
WITH FLUORESCENCE MICROSCOPY
Fluorescence microscopy can be used for the purposes of observing the organelles inside the yeast cells. This is particularly a great method through which students can get to view the intracellular distribution of the cell and identify the different types of cell organelle. However, this may prove a little challenging when it comes to yeast given that they are very small in size (compared to other cells) and have a cell wall (Chalfie and Kain, 2005).
ANOTHER METHOD
It is easy to distinguish them in direct mounts with lactophenol or simply water. Yeasts are usually much larger and mostly reproduce by budding. Some yeasts show filamentous structures (pseudohyphae or hyphae) in addition to single cells, while bacteria mostly appear as isolated round (cocci) or elongated (bacilli) elements (exceptions are the 'filamentous bacteria' such as Nocardia or Actinomadura, but they form very thin filaments without strong constrictions or septa),
Success!
HOPE YOU LIKE MY ANSWER IF YOU LIKE MARK ME AS BRAINLIEST
THANKYOU