How can you separate mixture of two Organic compounds by TLC method?
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The methods of isolating pure samples of the components from a mixture may be either physical or chemical. The physical method consists of fractional distillation and is applicable only if there is a wide difference between the boiling points of the two compounds and provided that an azeo-trope is not formed.
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I have mixture of two organic compounds one with molecular weight 226.41g/mol and other is 166.22 g/mol. Can I use size exclusion chromatography for the difference of 60 g/mol? Kindly do let me know how to separate.
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