Question

How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?

Answer 1

Hello, friend

1, Preparation of DNA corresponding A and B chain separately

2, extraction of plasmid from bacteria

3, insertion of DNA corresponding A and B chain separately plasmid

4, Transforming the bacteria with this recombinant plasmid

5, Expression of the desired product from this DNA

6, Purification of A and B chain

7, Linkthem disulfide bond

please mark BRAIN LIST