How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
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1, Preparation of DNA corresponding A and B chain separately
2, extraction of plasmid from bacteria
3, insertion of DNA corresponding A and B chain separately plasmid
4, Transforming the bacteria with this recombinant plasmid
5, Expression of the desired product from this DNA
6, Purification of A and B chain
7, Linkthem disulfide bond
please mark BRAIN LIST
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