how did you prepare semipermeable membrane in
the laboratory 8 to 10 sentences
Answers
To perform these activities following materials are required:-
1. Three beaker, 2 egg membrane, sugar, water , thread , measuring jar, disposable syringe, cloth and table spoon.
2. take the egg that are stoked in the dilute HCl.
3. wash the egg under tap water and wipe them with cloth.
4. Now remove the internal contain present in the egg. membranes carefully by making a pencil size hole in them. and wash membrane at fresh water.
5. now take one egg membrane and fill it with 10 ml of saturated sugar solution by using syringe.
6. next tie the mouth of the membrane with help of a thread.
7. now take a Bekar containing 100 ml of tap water and dip the membrane into it.
8. leave it aside for one night.
9. next take the second membrane and fill it with 10 ml of tap water with the help or syringe and ite its mouth with he help of thread.
10. take another bekar containing 100 ml of saturated sugar solution. and placed the second membrane inside this bekar.
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I hope it's help you.....!!!!
Materials
3 eggs
3 glasses (large enough to fit the egg plus liquid)
3 butter knives
White vinegar (about 3 cups)
Distilled water (about 2 cups)
Light corn syrup (about 1 ¼ cups)
Slotted spoon
Measuring cup (1 cup)
Measuring spoons (1 tablespoon and ½ tablespoon)
Sticky notes and marker
Scale (optional)
Procedure
Note: It’s okay to touch the eggs, but remember to wash your hands afterwards to avoid any nasty surprises!
Place one egg in each glass. Pour in enough vinegar to cover each egg. Bubbles will start to form around the egg, and it’ll float up. To keep it submerged, put a butter knife in the glass to hold it down.
Put the three glasses in the refrigerator and allow to sit for 24 hours.
Gently holding the egg in the glass, pour out the old vinegar. Replace with fresh vinegar, and let sit in the refrigerator for another 24 hours. Repeat this process until the shells are fully dissolved and only the membrane remains. This should take about 2-3 days.
Gently remove the eggs using the slotted spoon and rinse with tap water in the sink. Rinse out the empty glasses as well.
Gently put the shell-less eggs aside for a moment on a plate.
Prepare three different sugar-water solutions as follows, labeling with sticky notes:
Glass 1: Label “hypertonic”. Pour in one cup of corn syrup.
Glass 2: Label “isotonic”. Add 1 ½ tablespoons corn syrup to the one cup measuring cup, and fill the remainder with distilled water. Pour into glass (make sure you get all the corn syrup out!) and stir to dissolve.
Glass 3: Label “hypotonic”. Pour in one cup of distilled water.Gently put one shell-less egg in each of the glasses, and let sit in the refrigerator for another 24 hours.
osmosis-graph
Remove the glasses from the refrigerator, and gently put the eggs on a plate. If you weighed the eggs before putting them in each solution, weigh them again. What happened to each of the eggs?
egg-experiment-3
How does osmosis work?
Osmosis is the scientific term that describes how water flows to different places depending on certain conditions. In this case, water moves around to different areas based on a concentration gradient, i.e. solutions which have different concentrations of dissolved particles (solutes) in them. Water always flows to the area with the most dissolved solutes, so that in the end both solutions have an equal concentration of solutes. Think about if you added a drop of food dye to a cup of water – even if you didn’t stir it, it would eventually dissolve on its own into the water.
In biological systems, the different solutions are usually separated by a semipermeable membrane, like cell membranes or kidney tubules. These act sort of like a net that keeps solutes trapped, but they still allow water to pass through freely. In this way, cells can keep all of their “guts” contained but still exchange water.
Now, think about the inside of an egg. There’s a lot of water inside of the egg, but a lot of other things (i.e. solutes) too, like protein and fat. When you placed the egg in the three solutions, how do you think the concentration of solutes differed between the inside of the egg and outside of the egg? The egg membrane acts as a semipermeable membrane and keeps all of the dissolved solutes separated but allows the water to pass through.
How did osmosis make the eggs change size (or not)?
If the steps above work out properly, the results should be as follows.
In the case of the hypertonic solution, there were more solutes in the corn syrup than there were in the egg. So, water flowed out of the egg and into the corn syrup, and as a result the egg shriveled up.
In the case of the isotonic solution, there was roughly an equal amount of solutes in the corn syrup/water solution than there was in the egg, so there was no net movement in or out of the egg. It stayed the same size.
In the case of the hypotonic solution, there were more solutes in the egg than in the pure water. So, water flowed into the egg, and as a result, it grew in size.