Science, asked by sanju659, 1 year ago

how different pigments from an extract of flower petals can be separated by chromatography ​

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Answered by abhijeetgorai836
33

Answer:

The pigments from an extract of flower petal can be separated out by the process of chromatography. Chromatography is a technique of separation of substances which are very close in their molecular weight but differ in their molecular sizes. Since the pigments in flower petals have different molecular sizes so we can easily separate it out by the process of chromatography.

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Answered by Rameshjangid
0

The pigments from an extract of flower petal may be separated out with the aid of using the procedure of chromatography. Chromatography is a method of separation of materials which might be very near of their molecular weight however range of their molecular sizes. Since the pigments in flower petals have exceptional molecular sizes so we will effortlessly separate it out with the aid of using the procedure of chromatography.

  • Four number one pigments of inexperienced flora can effortlessly be separated and recognized the use of a method known as paper chromatography. These pigments consist of greenish pigments known as chlorophylls and yellowish pigments known as carotenoids. Pigments are separated consistent with variations of their relative solubilities.
  • In order to extract those pigments from the thylakoid membranes of the chloroplasts, the organelles wherein photosynthesis occurs, sparkling, floor or torn leaves (ideally spinach) can be soaked in acetone or focused alcohol. The chloroplast pigment extract pictured at left became received with the aid of using boiling sparkling leaves of spinach in 95% ethanol for numerous mins after which filtering the use of gravity filtration.
  • Pigments are then "painted" onto strips of chromatography paper with V-formed pointers the use of a small, hole glass tube or a small paintbrush. For great results, permit the road of pigments to dry, then repeat the procedure till a darkish inexperienced line of pigments is evident (approximately six instances is enough to reap a darkish pigment line).

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