How DNA and DNA hybridization help in taxonomic identification? ( 5 marks )
Answers
Answer:
Restricted fragment length polymorphism (RFLP)
Explanation:
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Answer:
DNA-DNA hybridization helps in determining the similarity in genetics which in turn helps in taxonomic identification.
Explanation:
DNA-DNA hybridization is a technique in molecular biology that helps in determining the amount of similarity between two DNA sequences.
First of all, the DNA of one organism is labeled. The labeling can be done using any radioisotope or fluorescent dyes. The labeled DNA of one organism is mixed with the unlabeled DNA of another organism. These two DNAs are mixed in order to examine the level of similarity between them.
When the DNAs are mixed they form new DNA sequences which are known as Hybridized sequences. Since DNA has a tendency to form double strands, the hybridized sequences will bind to each other.
When there is a high degree of similarity between the hybridized sequence, they will bind more firmly and will require more energy to separate them.
Thus, the hybridized sequences that bind firmly shows that they have similarity and are from a similar taxonomic group. The sequences that do not bind show that they are from different taxonomic groups.
In this way, DNA-DNA hybridization helps in taxonomic identification.