Question

how do cells aggregate together into tissues​

Answer 1

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To achieve this objective, the simplest way is by coating a surface with specific hydrophobic biomaterials to avoid cell adhesion. In this case, cells are induced to grow together as a 3D aggregation. The cells will first grow together and then attain a larger size by proliferation.

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Answer 2

Answer:

the simplest way is by coating a surface with specific hydrophobic biomaterials to avoid cell adhesion. In this case, cells are induced to grow together as a 3D aggregation. The cells will first grow together and then attain a larger size by proliferation. The most widely used material is agar. Other substrates, such as agarose gel and matrigel, can also be used to prevent adherence by using the same principle of inhibiting meaningful contact with the culture containers’ surface

Besides culturing cells over the coated surface, the spinner flask is also one of the most widely used methods. As a result of fluid movement, the spinner flask can avoid cells contacting the substrates, and also improve the spheroid’s nutrient penetration. Last but not least, the property of impact will be increased, which either can reduce the duration of cell aggregation or produce greater numbers of spheroids. There are some other approaches such as roller tubes and gyratory shakers that use the similar principle. However, most of them cannot control the numbers of cells per spheroid. To improve the distribution, hanging-drop method is applied to a broad range of cell lines successfully. Compared with growth on nonadherent surfaces, the coefficient of variation for HepG2 spheroids size is reduced from 40–60% to 10–15% and they attain a round shape when using the hanging-drop method

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