How do I make my cell phone supernatant free?
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The cells, after 20-24 hrs of culture, are removed by centrifugation at 15,000 rpm in a Sorvall centrifuge and the cell-free supernatant obtained. B. Three methods have been used for concentrating active serum-free supernatants - freeze drying, concentration in colloidin bags, and “diaflo” membrane filtration.
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