How do restriction endonucleases work?
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Like all enzymes, a restriction enzyme works by shape-to-shape matching. When it comes into contact with a DNA sequence with a shape that matches a part of the enzyme, called the recognition site, it wraps around the DNA and causes a break in both strands of the DNA molecule.
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Restriction Endonuclease
The enzyme which cuts the DNA strand at the ends is known as restriction endonuclease. They have specific recognitipn sites on the DNA strans characterized by some specific base pairs. It first suspects the entire strand of the DNA and once it find its recognition site, it binds to the DNA and cuts the two opposite strands. These recognition sites are known as palindromic sequence. It cuts the DNA strand a little away from the palindromic sequence. It cuts the opposite strands between the same two base pairs which results in the formation of overhanging stretches called Sticky Ends.
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