Question

How do restriction enzyme cut at specific sequences?

Answer 1

Restriction enzyme has specific palindromic sequence (about 6 base pairs)

So it cut the peace of DNA at that sequence....

Answer 2

Restriction Endonuclease

》Restriction Endonuclease is the enzyme which cuts the DNA at specific sequences.

》They consists of specific recognition sites which are characterised by some base pairs.

》It first suspects the entire strand of the DNA and once it find its recognition site, it binds to the DNA strand and cuts the two opposite strands.

》These recognition sites are termed as palindromic sequence.

》 This enzyme cuts the DNA strand a little away from the palindromic sequence.

》It cuts the opposite strands of the DNA between the same two base pairs which leads to the formation of overhanging stretches called Sticky Ends.