Question

how do we physically identify protein without test rxns?​

Answer 1

Explanation:

There are two methods that are commonly used to identify proteins: Edman Degradation and Mass Spectrometry. ... Peptide mass fingerprinting uses the masses of proteolytic peptides as input to a search of a database of predicted masses that arise from digestion of a list of known proteins.

The presence of protein is tested by the Biuret test for proteins. The Biurette reagent made of sodium hydroxide and copper (II) sulphate helps in determining the presence of protein in a sample.

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