How do you analyze western blot data?
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The data produced with a Western blot is usually quite easy to interpret. In the majority of cases, bands corresponding to the target protein will become visible upon treatment of the blot with substrate. Their identity is confirmed by comparison to molecular weight markers (for size) and a positive control (size and signal).
In some cases the data may be more complex, showing unexpected sizes, multiple bands, or alteration in bands following a particular treatment. The focus of this chapter is analysis and as such the data itself will be discussed along with examples of different types of Western blot data from research and clinical settings to demonstrate the flexibility of the technique.
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In some cases the data may be more complex, showing unexpected sizes, multiple bands, or alteration in bands following a particular treatment. The focus of this chapter is analysis and as such the data itself will be discussed along with examples of different types of Western blot data from research and clinical settings to demonstrate the flexibility of the technique.
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