how do you calculate percentage of metabolic activity with alamar blue assay
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I would like to calculate percentage of metabolic activity of bacteria with alamar blue assay.
for this, i have taken absorbance at OD600 and OD550 from wells with PBS + alamar blue, bacteria in LB + alamar blue, bacteria in PBS + alamar blue (bacteria need to be re suspended in PBS before treatment so this is our sample of interest).
now, is there any formula that I can use to calculate percentage of metabolic activity in bacterial suspension treated with different doses of chemical
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