Question

How do you correlate the base composition of DNA with that of melting temperature? the base composition of DNA can be determined?​

Answer 1

Explanation:

The melting temperature of a DNA(T

m

) is that temperature at which 50% of double-stranded DNA changes to single-stranded DNA. The T

m

depends on the guanine-cytosine(GC) content of the DNA. Thus, the DNA having least GC content will have least T

m

while the DNA having maximum GC will melt at a higher temperature. Considering the options given option A has least GC content of all four options.

So, the correct answer to the question is '5'-AATAAAGC-3'