How do you observe cell membrane in a peel of rheo leaf under microscope? draw the diagram of it? write your observations
Answers
Answer:
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Explanation:
Let us perform a simple experiment to study in detail about the process of plasmolysis in the plant cell.
Aim
To study the phenomena of plasmolysis in epidermal peels of Rhoeo plant leaves in hypotonic and hypertonic solutions using salt solution.
Theory
What is Plasmolysis?
It is a process of contraction or shrinkage of the protoplasm of a plant cell due to loss of water from the cell. It takes place in extreme conditions and hence occurs rarely. Plasmolysis can be carried out in a laboratory by submerging a living cell in a concentrated sugar or salt solution for water loss from the cell.
Why are Rheo plant’s leaves are used in this experiment?
The Rheo plant’s leaves are used in this experiment because the Rheo leaf has coloured cell sap, which can be examined clearly under a compound microscope.
How does water permeate through the cell membrane?
The cell membrane serves as a semipermeable membrane dividing the inner of all cells from that of its surroundings. This membrane permits movement of a few of the particles including, water molecules, ions across the membrane while blocking others. There is the continuous movement of water molecules in and out of the cell across the cell membrane ad also it serves as an important attribute for enabling cells to absorb water.
Materials Required
Needle.
Forceps.
Droppers.
Glass slides.
Watch glass.
Rhoeo leaf.
Cover slips.
Compound microscope.
Sodium chloride 5% solution.
Sodium chloride 0.1% solution.
Procedure
Take two clean and dried glass slides and place them on a table.
Select the fresh and cleaned Rhoeo leaf and place them on the watch glass.
Fold the leaves in such a way that it tears from the lower side of the leaf. Or, with the help of a clean blade.
Extract two small fragments of a fine and transparent layer with the help of forceps from the lower surface of the epidermis of the Rhoeo leaf.
Now set up the epidermal peels on each of the glass slides.
With the help of a dropper, add 1 to 2 drops of sodium chloride 0.1% solution on one of the prepared slide.
With the help of another dropper, add 1-2 drops sodium chloride 5% solution on the other prepared slide.
Now carefully set a cover slip on the peel of both sides with the help of a needle. Make sure, no bubbles are present.
Leave the prepared glass slide undisturbed for a few minutes.
Now carefully place the slides under a compound microscope and observe the changes.
Observation
After a period of 30 minutes, we can notice that cells placed in the sodium chloride 0.1% solution seem to be turgid and the cells placed in the sodium chloride 5% solution seem to be shrunk with the loss of water and it exhibits the process of plasmolysis.
Conclusion
Plasmolysis is observed when the plant cells are immersed in the concentrated salt solution or sodium chloride 5% solution. During this process, 4 to 5 per cent of water passes through the cell membrane into the encircling medium. This occurs as the concentration of water inside the cell is higher than the outside of the cell hence the protoplasm induces shrinkage and takes a spherical shape.
When the plant cells are immersed in a dilute salt solution or sodium chloride 0.1% solution, the water in the plant cells moves from the outside to the inside of the cell as the water concentration is higher outside the cell as compared to the inside of the cell which causes the turgidity of the cell.
