How does b-galactosidase coding sequence act as a selectable marker? Explain.
Answers
Answer:
The coding sequence of beta-galactosidase is considered as a selectable marker because the marker is present in the antibiotic resistance gene, due to which the antibiotics' inactivation occurs. This helps in the selection of recombinants.
Explanation:
Explanation: b galactosidase gene encodes for the enzyme beta galactosidase (obviously). when a foreign gene is inserted into this gene, the production of the enzyme is stopped. now the bacterial colonies are grown on chromogenic substrate. the colonies of bacteria which have taken up the recombinant DNA will produce no color as the the enzyme b galactosidase is absent but the the colonies which have not taken up the recombinant DNA will produce a blue color making it easy for us to separate the two colonies.