Question

How does column diameter affect column chromatography?

Answer 1

Answer:

This is due to there being an increased analyte concentration in the mobile phase. A change in column diameter will affect the loading capacity, both the volume of sample and the mass of analyte which can be injected onto the column, along with the flow rates which can be used.

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Answer 2

Larger diameter columns require higher flow rates, and thus larger volumes of mobile phase, to reach the desired linear velocity. ... By scaling down to a 3.2mm ID column, we can significantly reduce the flow rate and solvent volume needed to reach the same optimal linear velocity, without increasing run time.