Question

how does wavelength of light affects magnification and resolution of a light microscope?

Answer 1

Answer: The magnification of the microscope is inversely proportional to the wavelength of the light. The wavelength can be affected when the change the objective lens and the specimen. The visible light range in nanometers should be from 380nm to 750nm. The measured that magnification by multiples, such as 2x, 4x and 10x.

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Answer 2

The highest resolution power in optical microscopy is achieved by near-ultraviolet light, the shortest effective wavelength of imaging.

Explanation:

• The wavelength of light is an important factor for the resolution of the microscope. Shorter wavelengths give higher resolution.
• The highest resolution power in optical microscopy is achieved by near-ultraviolet light, the shortest effective wavelength of imaging.
• Near-ultraviolet light is followed by blue, then green, and finally red light with the ability to resolve the detail of the specimen.
• In most conditions, microscopists use white light generated by a tungsten halogen bulb to illuminate the specimen.
• The visible light spectrum is centered around 550 nanometres, the dominant wavelength for green light (our eyes are most sensitive to green light).

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