How elisa and pcr are different
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Whilst PCR-ELISA cannot provide an accurate estimation of the actual gene of interest that is present compared to real-time PCR (qPCR), it provides a quick summary of whether a particular substrate is high or low at a particular time, as colorimetric detection is in direct proportion to the number of the intended gene ..
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Compared to ELISA, real-time PCR showed greater agreement among duplicate samples. ELISA was found to be less time consuming and easier to perform than real-time PCR. ELISA and real-time PCR showed 100% specificity during reference sample testing
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