Question

how fixed tissue is prepared​

Answer 1

Frozen tissues are prepared by immersing the tissue in liquid nitrogen, isopentane or by burying the sample in dry ice. ... The frozen tissue sections may then be fixed, typically with an alcohol such as methanol or ethanol. As alcohols do not mask epitopes, their use avoids the need for antigen retrieval.

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Answer 2

Answer:

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Frozen tissues are prepared by immersing the tissue in liquid nitrogen, isopentane or by burying the sample in dry ice. ... The frozen tissue sections may then be fixed, typically with an alcohol such as methanol or ethanol. As alcohols do not mask epitopes, their use avoids the need for antigen retrieval.