How fragmentation of of DNA is done at specific position in recombinant DNA technology?
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Fragmentation of Dna is done by specialized enzymes called restriction endonucleases. There 4 types of restriction endonucleases.
Many restriction endonucleases cut both the strands of DNA near recognition sites. As a result there is formation of blunt ends
Other method of cutting of DNA is the two strands of DNA are cut by restriction endonucleases at two different points. Such cuts are know as staggered cuts. This results into generation of protruding ends i.e. one strand of double helix extends few bases than other
Many restriction endonucleases cut both the strands of DNA near recognition sites. As a result there is formation of blunt ends
Other method of cutting of DNA is the two strands of DNA are cut by restriction endonucleases at two different points. Such cuts are know as staggered cuts. This results into generation of protruding ends i.e. one strand of double helix extends few bases than other
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