How is formaldehyde prepared in laboratory?
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The analysis is done using the EPA, "Method for the determination of aldehydes and ketones in ambient air using HPLC, method TO5".
2. Samples are prepared by adding formaldehyde and formaldehyde-dinitrophenylhydrazine
derivative standards at three concentration levels to 10 ml DHPH reagent and 10 ml
isooctane.
3. The samples are mixed and the isooctane layer is removed and saved.
4. The DNPH layer is then extracted with
methylene chloride-hexane.
5. The sample is mixed and spun and the methylene chloride-hexane levels removed and
mixed with the isooctane in step 3.
6. This sample is placed in the CentriVap and evaporated to dryness.
7. After evaporation, 5 ml methanol is added and the sample analyzed by HPLC.
2. Samples are prepared by adding formaldehyde and formaldehyde-dinitrophenylhydrazine
derivative standards at three concentration levels to 10 ml DHPH reagent and 10 ml
isooctane.
3. The samples are mixed and the isooctane layer is removed and saved.
4. The DNPH layer is then extracted with
methylene chloride-hexane.
5. The sample is mixed and spun and the methylene chloride-hexane levels removed and
mixed with the isooctane in step 3.
6. This sample is placed in the CentriVap and evaporated to dryness.
7. After evaporation, 5 ml methanol is added and the sample analyzed by HPLC.
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