Question

How is human insulin synthesized using recombinant DNA technology?
A.By using chemically synthesized DNA sequences for the two chains separately
B. By isolating DNA from the islets of Langerhans of pancreas
C. By using cDNA for insulin
D. By using chemically synthesized DNA sequences for the complete insulin protein

Answer 1

Answer:

Recombinant DNA Technology is a process in which an enzyme is used to cut and paste together the DNA sequences that are required.

Explanation:

It is the technology used by scientists to insert the gene of a human into a common bacteria's genetic material. This is called "recombinant" This is used for treating diabetic patients.

cDNA is the DNA that is synthesized from a single-stranded RNA template which is catalyzed by an enzyme.

1. The gene which is responsible for producing human insulin is isolated.    
2. Then the plasmid of the bacteria is isolated.
3. Special enzymes are used to cut the plasmid.
4. The gene which is isolated is inserted into the bacteria through a plasmid which was cut before.
5. This process is called RECOMBINANT PROCESS.
6. Now, when the bacteria reproduce, it reproduces the human insulin genes as well.
7. This production of human insulin genes helps to treat diabetic patients.  

Hence, cDNA is used for insulin.