How is it determined if the desired dna with blunt ends is ligase in correct order?
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Blunt ends are generated by cutting both DNA strands in the middle of the recognition sequence. DNA ligase helps to join together the complementary ends ofinsert DNA and plasmid DNA. ... Most ofthese enzymes cut their recognition sequences at staggered sites, giving overhanging or cohesive single-stranded tails.
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Blunt ends are generated by cutting both DNA strands in the middle of the recognition sequence. DNA ligase helps to join together the complementary ends of insert DNA and plasmid DNA. ... Most of these enzymes cut their recognition sequences at staggered sites, giving overhanging or cohesive single-stranded tails.
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